Isolation of a cDNA encoding the human GM2 activator protein
نویسندگان
چکیده
منابع مشابه
Isolation of a cDNA encoding human 40S ribosomal protein s3.
A cDNA which encodes human 40S ribosomal protein s3 has been isolated from a lambda-Gem 2 cDNA library constructed using poly (A+) RNA from human WISH cells. The cDNA insert includes a 5' noncoding sequence of 22 nucleotides, an open reading frame of 729 nucleotides and a 3' noncoding sequence of 82 nucleotides followed by a 3' poly A tail. The hexameter AATAAA, which is the signal sequence for...
متن کاملTwo polymorphisms for the human GM2 activator protein gene.
Source/Description: A 300 bp HincII/EcoRI fragment of the human G^ activation protein cDNA (1) was subcloned into Bluescript vector and used as a probe. Polymorphism: Mspl identifies a two allele polymorphism with bands at either 3.4 kb (Al) or 2.9 kb (A2) and invariant bands at 1.0 kb, 1.8 kb and 4.0 kb. Rsal identifies a two allele polymorphism with a band at either 4.1 kb (Bl) or 3.4 kb (B2)...
متن کاملSequence of a cDNA encoding human ribosomal protein L26 and of a cDNA probably encoding human ribosomal protein L6.
Mammalian ribosomes consist of 82 different proteins and four RNA molecules. Sequence analysis of ribosomal proteins is essential for structural and functional studies and for understanding the evolution of ribosomes (1). In a search for genes related to non-P-glycoprotein mediated multidrug resistance in human lung cancer cell lines (2), we isolated three full-length cDNA clones corresponding ...
متن کاملA protein activator for the enzymic hydrolysis of GM2 ganglioside.
A protein activator (GMz-activator) specific for stimulating the hydrolysis of G M ~ ganglioside (GalNAcb1 4GalC3 + 2aNeuAc]/?l-+ 4Glcb1-+ 1’Cer) by &hexosaminidase A has been purified over 1OS-fold with a high yield from human liver. The purification procedure includes the adjustment of the pH of liver extract to pH 4.3, followed by ammonium sulfate precipitation, Sephadex G-200 filtration, an...
متن کاملIdentification and Molecular Characterization of the cDNA Encoding Cucumis melo Allergen, Cuc m 3, a Plant Pathogenesis-Related Protein
Background: Melon (Cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. To date, two allergen molecules, Cuc m 1 and Cuc m 2, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of Cuc m 3. Methods:The Cuc m 3 cDNA has been characterized by rapid amplification of cDNA ends (RACE), which ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1989
ISSN: 0014-5793
DOI: 10.1016/0014-5793(89)81454-1